The InviMag® Blood DNA Mini Kit/KF96 allows automated purification of genomic DNA from human blood samples, buffy coat, bone marrow and swabs using magnetic beads on the KingFisher™ Flex instrument. The kit can be used for fresh or frozen whole blood treated with EDTA or citrate, but not with heparin, from common blood collection systems.
|Downstream application||Molecular biology techniques like qPCR, NGS, hybridization methods and HLA typing|
|Target nucleic acid||Genomic DNA|
|Starting material||up to 200 µl: fresh or frozen blood (EDTA / citrate stabilized, but not heparinized)
up to 30 µl: buffy coat
up to 20 µl bone marrow
up to 200 µl rinsed liquid from swabs
|Yield||Whole blood: 3- 8 µg, depending on number of leukocytes, sample source, sample transport, sample storage, and age of the sample
|Quality||genomic DNA from Blood: A260:A280 1.6 – 2.1|
|Preparation time||54 min (incl. lysis)|
|Elution Volume||100 µl|
|Product Name||Package Size||Catalogue No.|
|InviMag® Blood DNA Mini Kit/ KF96||5 x 96 preparations||7431300200|
Frozen blood should be thawed in a 37°C water bath for 10–15 min, inverted carefully for several times and stored on ice until processing.
The average size of DNA fragments is 100-300 kb. The large fragments obtained are in general suitable for all common analytic applications like PCR techniques, hybridisation and NGS.
Elution Buffer M: 10 mM Tris pH 8.5 – 9