The InviMag® Blood DNA Mini Kit/KF96 allows automated purification of genomic DNA from human blood samples, buffy coat, bone marrow and swabs using magnetic beads on the KingFisher™ Flex instrument. The kit can be used for fresh or frozen whole blood treated with EDTA or citrate, but not with heparin, from common blood collection systems.
| Specifications | |
|---|---|
| Downstream application | Molecular biology techniques like qPCR, NGS, hybridization methods and HLA typing |
| Target nucleic acid | Genomic DNA |
| Starting material | up to 200 µl: fresh or frozen blood (EDTA / citrate stabilized, but not heparinized) up to 30 µl: buffy coat up to 20 µl bone marrow up to 200 µl rinsed liquid from swabs |
| Yield | Whole blood: 3- 8 µg, depending on number of leukocytes, sample source, sample transport, sample storage, and age of the sample |
| Quality | genomic DNA from Blood: A260:A280 1.6 – 2.1 |
| Preparation time | 54 min (incl. lysis) |
| Elution Volume | 100 µl |
| Technology | Magnetic beads |
| Certification | RUO |
24 MAY 2023
24 MAY 2023
24 MAY 2023
24 MAY 2023
24 MAY 2023
| Product Name | Package Size | Catalogue No. |
|---|---|---|
| InviMag® Blood DNA Mini Kit/ KF96 | 5 x 96 preparations | 7431300200 |
Frozen blood should be thawed in a 37°C water bath for 10–15 min, inverted carefully for several times and stored on ice until processing.
The average size of DNA fragments is 100-300 kb. The large fragments obtained are in general suitable for all common analytic applications like PCR techniques, hybridisation and NGS.
Elution Buffer M: 10 mM Tris pH 8.5 – 9
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