InviScreen® Norovirus Detection Kit provides a real-time RT-PCR method for the qualitative detection of norovirus genogroups I (GI) and II (GII) in food samples. The method is based on ISO 15216 and detects specific regions in the conserved 5′ end of the ORF1-ORF2 junction. The method is applicable to test samples of foodstuffs and surface swabs. The kit includes Infectious Bronchitis Virus (IBV) as a process control virus and its respective primer and probe sets, according to the ISO standard. The detection of amplified DNA fragments is achieved using different fluorescence channels (FAM, HEX/VIC and Cyanine 5) available in common PCR thermocyclers. Under optimal conditions, the kit provides highly sensitive detection of at least 10 copies of norovirus GI and GII.
| Specifications | |
|---|---|
| Sample preparation | For RNA extraction the use of RTP® Pathogen Kit (Art. No. 1050100300) or InviSorb® Spin Universal Kit (Art. No. 1040500300) is recommended. For this application, these products are classified as "for research use only". |
| LOD (Detection Limit) | 10 copies/µL |
| Specificity | Norovirus GI and GII |
| No. of tests | 100 |
| Technology | RT-qPCR / Real-Time RT-PCR |
| Instrument compatibility | Applied Biosystems™ 7500, Agilent AriaMx™, Bio Rad CFX96 Touch™ and CFX96 Touch™ Deep Well |
| Dyes | Channels Target: FAM, HEX and Cy5 |
| Certification | RUO |
12 OCT 2023
29 FEB 2024
| Product Name | Package Size | Catalogue No. |
|---|---|---|
| InviScreen® Norovirus Detection Kit | 100 reactions | 6015002200 |
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