PSP® SalivaGene DNA Kit
Item No.: 1035200200 & 1035200300
.png?h=1589&iar=0&w=2548)
Highlights:
- Flexible sample management: Kit can be combined with stabilized samples from saliva or swabs
- Isolation of bacterial DNA and host DNA
- High purity of DNA: removal of all PCR inhibitors
- CE-IVD certified, recommended for in vitro diagnostic use
.png?h=1589&iar=0&w=2548)
Product Details
The PSP® SalivaGene DNA Kit is perfect for oral microbiome analysis or the extraction of host DNA for genetic testing applications. The extraction kit is optimized for nucleic acid isolation of samples collected with the SalivaGene Collector or the SalivaGene Swab Comfort for comprehensive sample management – sampling, stabilization, transport, and storage.
| Specifications | |
|---|---|
| Downstream application | PCR techniques, NGS, hybridization methods and HLA typing |
| Target nucleic acid | Bacterial DNA, genomic host DNA |
| Starting material | SalivaGene Collector, SalivaGene Swab Comfort: 500 µl stabilized sample |
| Yield | Saliva: up to 10 µg Swabs: up to 1 µg (depending on sample material and donor) |
| Quality | A260: A280 1.6 – 2.1 |
| Preparation time | approx. 30 min (incl. lysis) |
| Elution Volume | 50 - 100 µl |
| Technology | Spin column |
| Certification | CE-IVD (in compliance with REGULATION (EU) 2017/746 on in vitro diagnostic medical devices) |
Downloads
Ordering Details
| Product Name | Package Size | Catalogue No. |
|---|---|---|
| PSP® SalivaGene DNA Kit | 50 purifications | 1035200200 |
| PSP® SalivaGene DNA Kit | 250 purifications | 1035200300 |
FAQs
-
How does DNA need to be stored after extraction?
For 2-6 months genomic DNA can be stored at +2 to +8°C. For long term storage keep genomic DNA at -15 to - 25°C or -70°C to -80°C. Avoid repeated freeze-thaw cycles as this may cause shearing of the DNA.
-
How do I perform a DNA precipitation to concentrate my sample?
Add 1/10 volume of 3M NaAcetate pH 5.2 and 2 - 2.5 volumes of ice cold 100% ethanol. Mix and store at -20°C for >1 hr to precipitate the DNA. Recover the DNA by centrifugation at full speed in a microcentrifuge for 15-20 min. Pour off the ethanol and wash the pellet twice with 70% ethanol at room temperature. Allow the pellet to air dry and resuspend the DNA in an appropriate volume of TE buffer or water.
-
What is the composition of Elution Buffer M?
Elution Buffer M: 10 mM Tris pH 8.5 – 9


