Custom DNA Oligo Synthesis
For qPCR, PCR and NGS
GMP-compliant oligos, verified by ESI-MS, with fast turnaround and full customization
- Oligos produced in GMP-compliant cleanroom facilities
- 100% ESI-MS verified before shipment
- Synthesis scales from 10 nmol to 1 µmol
- Wide range of modifications and purification options (desalting, Cartridge purification, RP-HPLC)
- Typical lead time: 3–7 business days (depending on scale and complexity)
Primer specifications & scales
Choose the scale that best fit your application. Yields shown are typical final yields after purification.
| Scale | Length (Bases) | Yield |
|---|---|---|
| 10 nmol | 15–35 | ≥ 5 nmol |
| 25 nmol | 15–60 | ≥ 12 nmol |
| 50 nmol | 12–90 | ≥ 20 nmol |
| 100 nmol | 10–90 | ≥ 35 nmol |
| 200 nmol | 6–100 | ≥ 50 nmol |
| 1 µmol | 6–199 | ≥ 150 nmol |
Need larger scales or special formats (e.g. plates, mixed oligos)? Contact us for project-specific manufacturing.
Available modifications
Customize your oligos to fit your assay design. We support a wide range of 5’ and 3’ modifications, backbone chemistries, and internal labels.
| Category | Examples |
|---|---|
| 5' Fluorophores | FAM / HEX / CalFluor Red 610 / Cy5 / Cy3 / JOE / VIC / ROX (NH ESTER) / Cy5 (NH ESTER) |
| 5' Modifications | Biotin / Amino / Phosphorylation |
| 3' Modifications | BHQ1 / BHQ2 / BHQ3 / TAMRA / SPACER C3 / SPACER C9 / SPACER C18 / MGB |
| Backbone modification | Phosphorothioate Bond (PTO) |
| Base/sugar modifications | Inosine / LNA Bases / O-Methyl Bases |
If you don’t see your preferred dye or modification, we can often source it on request.
Quenchers & compatible fluorophores
We offer the following quencher–fluorophore combinations commonly used in qPCR assays.
| Quencher | Compatibility (Fluorophores) |
|---|---|
| BHQ1 | FAM / HEX / JOE / VIC / Cy3 |
| BHQ2 | HEX / JOE / VIC / Cy3 / Cy5 / CalFluor Red 610 / ROX (NH ESTER) / Cy5 (NH ESTER) |
| BHQ3 | Cy5 |
| TAMRA | FAM / HEX / JOE / VIC |
| MGB | FAM / HEX / JOE / VIC / Cy3 / Cy5 |
Alternative dyes and equivalents
Need a specific platform-compatible dye? We offer alternatives to popular fluorophores (FAM, JOE, HEX, VIC, Cy3, Cy5), often compatible with major qPCR instruments.
| Base Fluorophore | Alternatives |
|---|---|
| FAM | ATTO 465 / Alexa 488 / Cy2 / Oregon Green |
| JOE / HEX / VIC | Alexa 532 / CAL Fluor Orange 560 / Bodipy 530/550 / TET / Yakima Yellow |
| Cy5 | ATTO 647N / ATTO 655 / LC 670 / DyLight 647 / Alexa Fluor 647 / TYE 705 |
| Cy3 | ATTO 565 / Alexa Fluor 555 / TYE 563 |
How to order custom oligos
Order the way that works best for your lab – upload a templated datasheet or place orders directly online.
Order via datasheet
Download the order template, fill in your sequences, scales, purification and modifications, and attach it to your purchase order.
Order Online
Use our online ordering platform to configure oligos, see pricing, and get an estimated delivery date instantly.
Need advice on assay design or dye/quencher selection?
Our specialists are available to review your design and recommend the optimal oligo setup.
FAQ - Custom Oligonucleotides General & Ordering