The InviScreen® O157:H7 Detection Kit is part of the complete workflow for the detection and identification of STEC E. coli in food samples. In combination with the InviScreen® STEC Detection Kit, this assay detects the presence of genes associated with O157:H7 serotype. The method uses the real-time PCR technique to detect the presence of the rfbE and fliC genes which code the virulence marker and its H-antigen-associated gene, which are specific of the O175:H7 serotype. The method is applicable to products intended for human consumption and the feeding of animals, environmental samples in food production and food handling, and environmental samples in primary production.
| Specifications | |
|---|---|
| Sample preparation | For DNA extraction the use of InviPrep® Fast Lysis Buffer (Art. No. 1001136100) or InviSorb® Spin Tissue Mini Kit (Art. No. 1032100300) are recommended |
| LOD (Detection Limit) | 3 genomic units |
| Specificity | STEC E. coli serotype O157:H7 |
| No. of tests | 50 |
| Technology | qPCR / Real-Time PCR |
| Instrument compatibility | Applied Biosystems™ 7500, Agilent AriaMx™, Bio Rad CFX96 Touch™ and CFX96 Touch™ Deep Well |
| Dyes | Channels Target: FAM and HEX/VIC |
| Certification | RUO |
15 JUN 2023
29 FEB 2024
| Product Name | Package Size | Catalogue No. |
|---|---|---|
| InviScreen® O157:H7 Detection Kit | 50 reactions | 6015003201 |
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