The InviSorb® Spin Blood Mini Kit is the ideal tool for fast and convenient purification of genomic DNA from human blood as well as from buffy coat. Fresh or frozen whole blood treated with EDTA or citrate, but not with heparin, from common blood collection systems can be used. A separation of leukocytes is not necessary. The kit is validated for leukocyte counts of 3x106 - 1x107cells/ml for high yields of up to 10 µg.
|Downstream application||Molecular biology techniques like qPCR, NGS, hybridization methods and HLA typing|
|Target nucleic acid||DNA|
|Yield||up to 10 μg (in average 6 μg), depending on number of leukocytes, sample source, sample transport, sample storage, and age of the sample|
|Quality||A260:A280 1.7 – 2.0
|Preparation time||20 min (incl. lysis)|
|Elution Volume||30 - 200 µl|
|Certification||CE-IVD (in compliance with REGULATION (EU) 2017/746 on in vitro diagnostic medical devices)|
|Product Name||Package Size||Catalogue No.|
Frozen blood should be thawed in a 37°C water bath for 10–15 min, inverted carefully for several times and stored on ice until processing.
Yes, this is possible as an RUO application. Note that bird (e.g., chicken) or fish blood contains nucleated erythrocytes. Therefore, only 10-15 µl of the starting material should be used for isolation.
The average size of DNA fragments is 20 – 30 kb, the maximum fragment size can be up to 50 kb. The average fragment size is in general suitable for all common analytic applications like PCR techniques, hybridisation and NGS.
Elution Buffer M: 10 mM Tris pH 8.5 – 9