Invisorb® Fragment CleanUp can be used for clean-up of PCR products and other enzymatic reactions, as well as for the up-concentration of sample volumes. In addition, the kit can be used for the purification of DNA fragments from agarose gels. DNA fragments from 80 bp - 30 kb can be purified from standard or low melting agarose gels in TAE and TBE buffers with high final DNA concentrations.
|Downstream application||Sequencing, cloning, ligation, restriction enzymes digestion, PCR|
|Target nucleic acid||DNA|
up to 100 µl reaction volume:
|Recovery||80 – 95 %, depends on fragment length (PCR clean-up)
60 - 90 %, depends on fragment length and type of agarose gel
|Size range||Fragment sizes of 80 bp – 30 kb can be purified|
|Preparation time||approx. 7 min (PCR clean-up)
approx. 20 min (agarose gel extraction)
|Elution Volume||10 - 50 µl|
|Product Name||Package Size||Catalogue No.|
|InviSorb® Fragment CleanUp||250 purifications||1020300300|
The InviSorb® Fragment CleanUp can be used for all standard agaroses in TAE or TBE buffer. An agarose concentration ranging from 0.8 % - 2.0 % is recommended for DNA fragmentation and subsequent purification.
Elution Buffer: 10 mM Tris pH 8.5 – 9