InviSorb® Fragment CleanUp

Item No.: 1020300300

Highlights:

Timesaving: Only 7 min for clean-up (2-step bind-and-elute protocol) or 20 min for agarose gel purification
Starting volume: up to 100 μl reaction mixture or up to
300 mg agarose gel pieces
Flexible use: PCR clean-up and agarose gel extraction, suitable for the up-concentration of samples, using a minimal elution volume of 10 μl
High recovery: 95% recovery for fragment sizes of
80 bp – 30 kb

Product Details

Invisorb^® Fragment CleanUp can be used for clean-up of PCR products and other enzymatic reactions, as well as for the up-concentration of sample volumes. In addition, the kit can be used for the purification of DNA fragments from agarose gels. DNA fragments from 80 bp - 30 kb can be purified from standard or low melting agarose gels in TAE and TBE buffers with high final DNA concentrations.

Specifications
Downstream application	Sequencing, cloning, ligation, restriction enzymes digestion, PCR
Target nucleic acid	DNA
Starting material	up to 100 µl reaction volume: PCR reaction mixture restriction digestion mixture ligation mixture cDNA synthesis mixture cycle sequencing reaction mixture up to 300 mg of gel slices (0.8 – 2%) from TAE or TBE agarose gels
Recovery	80 – 95 %, depends on fragment length (PCR clean-up) 60 - 90 %, depends on fragment length and type of agarose gel
Size range	Fragment sizes of 80 bp – 30 kb can be purified
Preparation time	approx. 7 min (PCR clean-up) approx. 20 min (agarose gel extraction)
Elution Volume	10 - 50 µl
Technology	Spin column
Certification	RUO

Resources

Ordering Details

Product Name	Package Size	Catalogue No.
InviSorb^® Fragment CleanUp	250 purifications	1020300300

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FAQs

From which kind of agarose can DNA fragments be purified with the InviSorb® Fragment CleanUp?

The InviSorb® Fragment CleanUp can be used for all standard agaroses in TAE or TBE buffer. An agarose concentration ranging from 0.8 % - 2.0 % is recommended for DNA fragmentation and subsequent purification.