The InviTrap® Spin Universal RNA Mini Kit simplifies total RNA isolation from different starting materials such as cells, tissue, and blood. In addition, RUO protocols are available for e.g., RNA Cleanup from TRIzol®, simultaneous extraction of DNA and RNA or RNA extraction from other body fluids. Genomic DNA is removed without an enzymatic digestion step, and RNases are inactivated to prevent RNA degradation.
|Downstream application||PCR techniques, NGS and hybridization methods|
|Target nucleic acid||RNA|
|Quality||A260: A280 1.8 – 2.3|
|Elution Volume||30 – 100 µl|
|Certification||CE-IVD (in compliance with REGULATION (EU) 2017/746 on in vitro diagnostic medical devices)|
|Product Name||Package Size||Catalogue No.|
|InviTrap® Spin Universal RNA Mini Kit||50 purifications||1060100200|
|InviTrap® Spin Universal RNA Mini Kit||250 purifications||1060100300|
The RNA concentration can be determined by measuring the absorbance at 260 nm (A260) in a spectrophotometer. Measured values should be higher than 0.10 to ensure significance. An absorbance of 1 unit at 260 nm corresponds to 40 μg RNA per ml (this ratio applies only to measurements at neutral pH). The ratio between absorbance values at 260 nm and
280 nm provides an estimate of RNA purity (see FAQ on RNA purity). Use the buffer in which the RNA is diluted to calibrate the spectrophotometer.
Purified RNA can be stored at -80°C and is stable for several years at this condition.
Elution Buffer R: RNase free water
|Cell line||Source||Yield [µg] from 1 x 105 cells|
|HeLa||human cervical carcinoma||2.0|
|Jurkat||human T-cell leukemia||1.7|