InviTrap® Spin Universal RNA Mini Kit

Item No.: 1060100200 & 1060100300

Highlights:

Pure RNA without DNase digestion - selective genomic DNA removal during lysis step
Flexible use: Optimized protocols for cells, tissue, and blood - incl. additional RUO protocols for RNA Cleanup from TRIzol^® phases and enzymatic reactions or simultaneous DNA/RNA isolation
CE-IVD certified, recommended for in vitro diagnostic use

Product Details

The InviTrap^® Spin Universal RNA Mini Kit simplifies total RNA isolation from different starting materials such as cells, tissue, and blood. In addition, RUO protocols are available for e.g., RNA Cleanup from TRIzol^®, simultaneous extraction of DNA and RNA or RNA extraction from other body fluids. Genomic DNA is removed without an enzymatic digestion step, and RNases are inactivated to prevent RNA degradation.

Specifications
Downstream application	PCR techniques, NGS and hybridization methods
Target nucleic acid	RNA
Starting material	Cell culture: up to 1 x 10⁷ cells/ml Up to 1.5 ml fresh whole blood (EDTA / citrate stabilized, but not heparin) Up to 20 mg fresh or frozen tissue
Yield	From cell culture: up to 80 µg total RNA e.g., 10 - 15 μg total RNA from 5 x 10⁵ NIH 3T3 fibroblasts From blood: up to 1 - 5 µg total RNA / ml of whole blood (depending on leukocyte count) From tissue: up to 80 µg total RNA (depending on type and amount of starting material)
Quality	A₂₆₀: A₂₈₀ 1.8 – 2.3
Preparation time	Cells: approx. 20 - 25 min (incl. lysis) Blood: approx. 45 min (incl. Buffer EL treatment) Tissue: approx. 15 - 20 min (excl. sample homogenization)
Elution Volume	30 – 100 µl
Technology	Spin column
Certification	CE-IVD (in compliance with REGULATION (EU) 2017/746 on in vitro diagnostic medical devices)

Resources

Ordering Details

Product Name	Package Size	Catalogue No.
InviTrap^® Spin Universal RNA Mini Kit	50 purifications	1060100200
InviTrap^® Spin Universal RNA Mini Kit	250 purifications	1060100300

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FAQs

How to quantify RNA?

The RNA concentration can be determined by measuring the absorbance at 260 nm (A260) in a spectrophotometer. Measured values should be higher than 0.10 to ensure significance. An absorbance of 1 unit at 260 nm corresponds to 40 μg RNA per ml (this ratio applies only to measurements at neutral pH). The ratio between absorbance values at 260 nm and
280 nm provides an estimate of RNA purity (see FAQ on RNA purity). Use the buffer in which the RNA is diluted to calibrate the spectrophotometer.

How should purified RNA be stored?

Purified RNA can be stored at -80°C and is stable for several years at this condition.

What is the composition of the Elution Buffer R?

Elution Buffer R: RNase free water

What average yield can be expected from different cell lines?

Cell line	Source	Yield [µg] from 1 x 10⁵ cells
HeLa	human cervical carcinoma	2.0
Jurkat	human T-cell leukemia	1.7
MRC 5	human	5.0
NIH3T3	human	6.5

InviTrap® Spin Universal RNA Mini Kit

Item No.: 1060100200 & 1060100300

Highlights:

Product Details

Resources

Instructions for use - InviTrap Spin Universal RNA Mini Kit_EN

Instructions for use - InviTrap Spin Universal RNA Mini Kit_DE

Instructions for use - InviTrap Spin Universal RNA Mini Kit_ES

Instructions for use - InviTrap Spin Universal RNA Mini Kit_FR

Instructions for use - InviTrap Spin Universal RNA Mini Kit_IT

Instructions for use - InviTrap Spin Universal RNA Mini Kit_PT

Ordering Details

FAQs

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