The PSP® SalivaGene DNA Kit is perfect for oral microbiome analysis or the extraction of host DNA for genetic testing applications. The extraction kit is optimized for nucleic acid isolation of samples collected with the SalivaGene Collector or the SalivaGene Swab Comfort for comprehensive sample management – sampling, stabilization, transport, and storage.
|Downstream application||PCR techniques, NGS, hybridization methods and HLA typing|
|Target nucleic acid||Bacterial DNA, genomic host DNA|
|Starting material||SalivaGene Collector, SalivaGene Swab Comfort: 500 µl stabilized sample|
|Yield||Saliva: up to 10 µg
Swabs: up to 1 µg
(depending on sample material and donor)
|Quality||A260: A280 1.6 – 2.1|
|Preparation time||approx. 30 min (incl. lysis)|
|Elution Volume||50 - 100 µl|
|Certification||CE-IVD (in compliance with REGULATION (EU) 2017/746 on in vitro diagnostic medical devices)|
|Product Name||Package Size||Catalogue No.|
For 2-6 months genomic DNA can be stored at +2 to +8°C. For long term storage keep genomic DNA at -15 to - 25°C or -70°C to -80°C. Avoid repeated freeze-thaw cycles as this may cause shearing of the DNA.
Add 1/10 volume of 3M NaAcetate pH 5.2 and 2 - 2.5 volumes of ice cold 100% ethanol. Mix and store at -20°C for >1 hr to precipitate the DNA. Recover the DNA by centrifugation at full speed in a microcentrifuge for 15-20 min. Pour off the ethanol and wash the pellet twice with 70% ethanol at room temperature. Allow the pellet to air dry and resuspend the DNA in an appropriate volume of TE buffer or water.
Elution Buffer M: 10 mM Tris pH 8.5 – 9