The InviPrep® Fast Lysis Buffer provides a fast and efficient solution for DNA isolation from gram-positive and gram-negative bacteria. It simplifies the extraction process, requiring minimal handling and delivering excellent results. DNA can be isolated from bacterial colonies or liquid bacterial culture such as selective media of pre-enriched food samples. With just a few simple steps, the Fast Lysis Buffer quickly lyses bacteria, removing potential inhibitors from the eluate. This ensures purified DNA of high quality, ready for downstream molecular biology applications like PCR or LAMP analysis.
|Downstream application||PCR techniques (real-time PCR, LAMP), sequencing, cloning|
|Target nucleic acid||DNA|
|Yield||Depending on sample type|
|Quality||Depending on sample type|
|Preparation time||~ 20 min for 24 samples|
|Elution Volume||50 µl|
|Technology||Fast Lysis Buffer|
|Product Name||Package Size||Catalogue No.|
|InviPrep® Fast Lysis Buffer||20 ml/bottle (for 100 preparations)
Yes, even though the buffer is officially specified for DNA extraction from gram pos. and gram neg bacteria, preliminary experiments have shown great potential to isolate DNA from samples like blood spots and yeasts. Please contact us for more information for your special experiments and needs.
Inhibitors are destroyed by the specific chemical composition of the buffer during sample lysis; in addition, the InviPrep® Fast Lysis Buffer contains components that effectively scavenge inhibitors. Thus, following centrifugation, the extract can be used directly for PCR or other assays.