The InviPrep® Fast Lysis Buffer provides a fast and efficient solution for DNA isolation from Gram-positive and Gram-negative bacteria as well as range of different fungi (yeast and mold). It simplifies the extraction process, requiring minimal handling and delivering excellent results. DNA can be isolated from bacteria and fungi in culture broth (e.g., pre-enriched food samples) or culture plate samples. In a few simple steps, the InviPrep® Fast Lysis Buffer rapidly lyses bacteria and fungi and removes potential inhibitors from the eluate. This ensures high-quality purified DNA ready for downstream molecular biology applications such as PCR or LAMP analysis.
| Specifications | |
|---|---|
| Downstream application | PCR techniques (real-time PCR, LAMP), sequencing, cloning |
| Target nucleic acid | DNA |
| Starting material |
|
| Yield | Depending on sample type |
| Quality | Depending on sample type |
| Preparation time | ~ 20 min for 24 samples |
| Elution Volume | 50 µl |
| Technology | Fast Lysis Buffer |
| Certification | RUO |
07 MAR 2024
29 MAY 2024
| Product Name | Package Size | Catalogue No. |
|---|---|---|
| InviPrep® Fast Lysis Buffer | 20 ml/bottle (for 100 preparations) |
1001136100 |
Yes, even though the buffer is officially specified for DNA extraction from gram pos. and gram neg. bacteria and fungi, preliminary experiments have shown great potential to isolate DNA from samples like blood spots. Please contact us for more information for your special experiments and needs.
Inhibitors are destroyed by the specific chemical composition of the buffer during sample lysis; in addition, the InviPrep® Fast Lysis Buffer contains components that effectively scavenge inhibitors. Thus, following centrifugation, the extract can be used directly for PCR or other assays.
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