The InviSorb® Spin Plant Mini Kit provides a fast and simple way to isolate high-quality DNA from plant species and tissue types. In addition, the kit can be used for the isolation of DNA from food and feed products of plant origin. The kit uses a prefiltration spin column, a unique filtration and homogenization column that efficiently removes cell debris and improves sample handling. This optimized procedure produces reliable results, making it an ideal solution for any laboratory working with plant DNA.
|Downstream application||PCR, RFLP analysis, Restriction Enzyme Digestion, Sequencing, Cloning, Southern Blotting|
|Target nucleic acid||DNA|
|up to 100 mg of plant material
up to 60 mg of dried plant material
|Yield||up to 50 µg, depends on amount and kind of starting material|
|Quality||A260: A280 1.6 – 2.0|
|Preparation time||20 min (without lysis)|
|Elution Volume||50 – 200 µl|
|Product Name||Package Size||Catalogue No.|
|InviSorb® Spin Plant Mini Kit||250 purifications||1037100300|
Yes, you can. Using the InviSorb® Spin Plant Mini Kit, the extraction of DNA has been performed successfully from species like Fucus vesiculosus (algae), Sphagnum sp. (bryophyta), Xerocomus badicus (fungi) and Cladonia sp. (lichens). Also here grinding of the starting material under liquid nitrogen the basis for a high yield.
Elution Buffer: 10 mM Tris pH 8.5 – 9
The InviSorb® Spin Plant Mini Kit co-purifies RNA to a certain extent. If it is necessary to eliminate the RNA, perform RNase A treatment as described in the IFU. Make sure, sample lysis is performed for 30 minutes at 65°C as described in the protocol, otherwise Proteinase S in the lysis mix still might be active and destroy RNase A. To inactivate Proteinase S, the lysis mix can be incubated at 80°C for some minutes.