InviScreen® IBDV Detection Kit provides a real-time PCR method for the qualitative detection of Infectious Bursal Disease Virus (IBDV) RNA in biological samples of animal origin. The method is based on a real-time reverse transcription and multiplex amplification strategy for the qualitative determination of the absence or presence of IBDV by detecting a region within the hypervariable region of VP2. The method is applicable to detect IBDV in tissues from Fabricius pouches collected by scraping the internal epithelial layer of the pouch using a sterile blade or swab. This kit includes an Internal Amplification Control (IAC) detection primer/probe set as a process control for the presence of PCR inhibitors. The real-time PCR method can be performed in commonly available PCR instruments with FAM and VIC/HEX fluorescence channels.
| Specifications | |
|---|---|
| Sample preparation | For RNA extraction the use of RTP® Pathogen Kit (Art. No. 1050100300) or InviSorb® Spin Universal Kit (Art. No. 1040500300) is recommended. For this application, these products are classified as "for research use only". |
| LOD (Detection Limit) | 100 copies/µL |
| Specificity | Infectious Bursal Disease Virus |
| No. of tests | 100 |
| Technology | qPCR / Real-Time PCR |
| Instrument compatibility | Applied Biosystems™ 7500, Agilent AriaMx™, Bio Rad CFX96 Touch™ and CFX96 Touch™ Deep Well |
| Dyes | Channel Target: FAM, Channel IAC: HEX |
| Certification | RUO |
09 FEB 2024
| Product Name | Package Size | Catalogue No. |
|---|---|---|
| InviScreen® IBDV Detection Kit | 100 reactions | 6016001200 |
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