The InviSorb® Spin Universal Kit simplifies workflows in the diagnostic laboratory. Use only one kit for the isolation of different nucleic acids from a variety of clinically relevant starting materials. Optimized protocols for specific sample types ensure reproducible results in infectious diseases and enable comprehensive multiplex analysis.
|Downstream application||Molecular biology techniques like qPCR, cDNA synthesis, microarray, Loop-mediated Isothermal Amplification (LAMP)|
|Target nucleic acid||Viral DNA & RNA, bacterial DNA, genomic DNA|
|Yield||Depending on sample (storage and source)
Whole blood: in average 1 µg DNA
Genomic DNA from Blood: A260:A280 1.8 – 2.1
Other sample types: depending on sample type, target nucleic acids
|Preparation time||Approx. 30 min for 12 samples (without lysis)|
|Elution Volume||100 – 200 µl|
|Certification||CE-IVD (in compliance with REGULATION (EU) 2017/746 on in vitro diagnostic medical devices)|
|Product Name||Package Size||Catalogue No.|
|InviSorb® Spin Universal Kit||50 purifications||1050100200|
|InviSorb® Spin Universal Kit||250 purifications||1050100300|
When different types of nucleic acids are present in a sample, you will get a co-purification of all of them. This means for example, if you are looking for a special viral nucleic acid in a human or animal sample you will also co-purify the genomic nucleic acid as well.
The Carrier RNA serves several purposes. It improves the reversible binding of viral nucleic acids to the RTA spin filter membrane. The addition of carrier RNA reduces the risk of viral nucleic acid degradation and minimises the binding of the viral nucleic acid to the reaction vessels.
Elution Buffer M: 10 mM Tris pH 8.5 – 9