The InviMag® Blood DNA Mini Kit/IG enables fully automated purification of genomic DNA from human blood samples on the InviGenius® PLUS with sample volumes of up to 200 µl. Fresh or frozen whole blood treated with EDTA or citrate, but not with heparin, from common blood collection systems can be used. Different primary tubes can be placed directly into the sample loading rack. A defined and optimized protocol provides high yields of pure DNA. Automatic barcode reading enables full tracking of samples and reagents.
|Downstream application||Molecular biology techniques like qPCR, NGS, hybridization methods and HLA typing|
|Target nucleic acid||Genomic DNA|
|Starting material||200 µl fresh or frozen human whole blood (EDTA / citrate stabilized, but not heparinized)|
|Yield||2-10 µg, depending on number of leukocytes, sample source, sample transport, sample storage, and age of the sample|
|Quality||A260:A280 1.6 – 2.0|
|Preparation time||approx. 90 min per run (12 samples)|
|Elution Volume||100 – 200 µl|
|Certification||CE-IVD (in compliance with REGULATION (EU) 2017/746 on in vitro diagnostic medical devices)|
|Product Name||Package Size||Catalogue No.|
|InviMag® Blood DNA Mini Kit/ IG||8 x 12 purifications||2431120100|
Frozen blood should be thawed in a 37°C water bath for 10–15 min, inverted carefully for several times and stored on ice until processing.
The average size of DNA fragments is 100-300 kb. The large fragments obtained are in general suitable for all common analytic applications like PCR techniques, hybridisation and NGS.
Elution Buffer: 10 mM Tris pH 8.5 – 9