The InviMag® Stool DNA Kit/ KF96 allows the convenient and automated purification of genomic and microbial DNA using magnetic beads on the KingFisher™ Flex instrument. The kit can be used for the extraction of stabilized samples or fresh/frozen stool samples. For comprehensive sample management – sampling, stabilization, transport and storage – the extraction kit can be combined with the Stool Collection Tubes with DNA Stabilizer.
|Downstream application||16S rRNA Microbiome profiling, Shotgun Metagenomic Sequencing, qPCR and arrays|
|Target nucleic acid||Bacterial DNA, genomic host DNA|
|Fresh or frozen stool samples: max. 200 mg
Stabilized stool samples (Stool Collection Tubes with DNA Stabilizer): 1.4 ml
|Yield||Up to 50 µg, depending on sample (storage and source)|
|Quality||A260: A280 1.6 – 1.8|
|Preparation time||about 20 -25 min after lysis|
|Elution Volume||150 µl (minimal volume 75 µl)|
|Product Name||Package Size||Catalogue No.|
|InviMag® Stool DNA Kit/ KF96||5 x 96 purifications||7438300200|
The average size of DNA fragments is 100-300 kb. The large fragments obtained are in general suitable for all common analytic applications like PCR techniques, hybridisation and NGS.
Elution Buffer: 10 mM Tris pH 8.5 – 9
For 2-6 months genomic DNA can be stored at +2 to +8°C. For long term storage keep genomic DNA at -15 to - 25°C or -70°C to -80°C. Avoid repeated freeze-thaw cycles as this may cause shearing of the DNA.