The InviMag® Stool DNA Kit/ KF96 allows the convenient and automated purification of genomic and microbial DNA using magnetic beads on the KingFisher™ Flex instrument. The kit can be used for the extraction of stabilized samples or fresh/frozen stool samples. For comprehensive sample management – sampling, stabilization, transport and storage – the extraction kit can be combined with the Stool Collection Tubes with DNA Stabilizer.
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| Specifications | |
|---|---|
| Downstream application | 16S rRNA Microbiome profiling, Shotgun Metagenomic Sequencing, qPCR and arrays |
| Target nucleic acid | Bacterial DNA, genomic host DNA |
| Starting material | Fresh or frozen stool samples: max. 200 mg Stabilized stool samples (Stool Collection Tubes with DNA Stabilizer): 1.4 ml |
| Yield | Up to 50 µg, depending on sample (storage and source) |
| Quality | A260: A280 1.6 – 1.8 |
| Preparation time | about 20 -25 min after lysis |
| Elution Volume | 150 µl (minimal volume 75 µl) |
| Technology | Magnetic beads |
| Certification | RUO |
15 AUG 2023
20 NOV 2023
30 JAN 2024
| Product Name | Package Size | Catalogue No. |
|---|---|---|
| InviMag® Stool DNA Kit/ KF96 | 5 x 96 purifications | 7438300200 |
The average size of DNA fragments is 100-300 kb. The large fragments obtained are in general suitable for all common analytic applications like PCR techniques, hybridisation and NGS.
Elution Buffer: 10 mM Tris pH 8.5 – 9
For 2-6 months genomic DNA can be stored at +2 to +8°C. For long term storage keep genomic DNA at -15 to - 25°C or -70°C to -80°C. Avoid repeated freeze-thaw cycles as this may cause shearing of the DNA.
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