The PSP® Spin Stool DNA Basic Kit is perfect for gut microbiome analysis or the extraction of host DNA. The kit can be used for the extraction of stabilized samples or fresh/frozen stool samples. For comprehensive sample management – sampling, stabilization, transport, and storage – the extraction kit can be combined with the Stool Collection Tubes with DNA Stabilizer. For direct lysis and extraction from fresh or frozen stool samples the PSP® Spin Stool DNA Basic Kit needs to be combined with the DNA Stabilizer available in bottle format.
|Downstream application||16S rRNA Microbiome profiling, Shotgun Metagenomic Sequencing, qPCR and arrays|
|Target nucleic acid||Bacterial DNA, genomic host DNA|
|Fresh or frozen stool samples: max. 200 mg
Stabilized stool samples (Stool Collection Tubes with DNA Stabilizer): 1.4 ml
|Yield||Up to 50 µg, depending on sample (storage and source)|
|Quality||A260: A280 1.4 – 1.8|
|Preparation time||approx. 45 min (incl. lysis)|
|Elution Volume||100 - 200 µl|
|Certification||CE-IVD (in compliance with REGULATION (EU) 2017/746 on in vitro diagnostic medical devices)|
|Product Name||Package Size||Catalogue No.|
|PSP® Spin Stool DNA Basic Kit||50 purifications||1038120200|
|PSP® Spin Stool DNA Basic Kit||250 purifications||1038120300|
For comprehensive sample management (sample collection, transport and storage at room temperature) we recommend combining the extraction kit with our Stool Collection Tubes with DNA Stabilizer (Cat.No 1038111300). If fresh or frozen pure sample is extracted combine the kit with the DNA Stabilizer (180 ml bottle, Cat. No. 1038111100). The DNA Stabilizer works as the lysis buffer for sample purification.
Elution Buffer: 10 mM Tris pH 8.5 – 9
For 2-6 months genomic DNA can be stored at +2 to +8°C. For long term storage keep genomic DNA at -15 to - 25°C or -70°C to -80°C. Avoid repeated freeze-thaw cycles as this may cause shearing of the DNA.