The InviScreen® Hepatitis A Virus Detection Kit provides a real-time RT-PCR method for the qualitative detection of Hepatitis A virus (HAV) in food samples. The method uses the real-time RT-PCR technique, following the ISO/TS 15216 specifications, with a primer set targeted at a conserved region across all HAV genotypes. The method is applicable to test samples of foodstuffs and surface swabs. The kit includes a process control virus and its respective primer and probe sets, according to the ISO standard. The detection of amplified DNA fragments is achieved using different fluorescence channels (FAM, HEX/VIC and Cyanine 5) available in common PCR thermocyclers. Under optimal conditions, the kit provides highly sensitive detection of at least 10 copies of HAV.
| Specifications | |
|---|---|
| Sample preparation | For RNA extraction the use of RTP® Pathogen Kit (Art. No. 1050100300) or InviSorb® Spin Universal Kit (Art. No. 1040500300) is recommended. For this application, these products are classified as "for research use only". |
| LOD (Detection Limit) | 10 copies/µL |
| Specificity | Hepatitis A virus |
| No. of tests | 100 |
| Technology | RT-qPCR / Real-Time RT-PCR |
| Instrument compatibility | Applied Biosystems™ QuantStudio Real-Time PCR Systems , Agilent AriaMx™, Bio Rad CFX96 Touch™ and CFX96 Touch™ Deep Well |
| Dyes | Channels Target: FAM, HEX/VIC and Cy5 |
| Certification | RUO |
26 FEB 2024
29 FEB 2024
| Product Name | Package Size | Catalogue No. |
|---|---|---|
| InviScreen® Hepatitis A Virus Detection Kit |
100 reactions | 6015006200 |
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