The technology behind our automated DNA and RNA extraction kits

Invitek Technologies - Non-Chaotropic Buffer System

Introduction

Nucleic acid extraction is a critical step in molecular biology workflows, serving as the foundation for downstream applications such as PCR, reverse transcription PCR (RT-PCR), next-generation sequencing (NGS), and other diagnostic and research methodologies. While manual extraction kits offer cost-effectiveness for small sample numbers and adaptability, automated extraction systems provide enhanced efficiency and consistency, meeting the demands of mid to high-throughput laboratories.

Magnetic Bead Technology

The InviMag® product line employs advanced magnetic bead technology for high-quality nucleic acid purification. This process follows the bind-wash-elute principle, similar to spin filter methods, but with a key distinction: nucleic acids bind to the surface of magnetic beads rather than a spin filter membrane.

  • Lysis: Nucleic acids are released from the sample.
  • Binding: Nucleic acids selectively bind to the magnetic beads.
  • Washing: Impurities and contaminants are removed, ensuring high purity.
  • Elution: Purified DNA and RNA are eluted from the beads using low-salt elution buffers or water, yielding high-purity nucleic acids with excellent recovery rates.

Versatility: InviMag® kits are compatible with various sample types and most magnetic bead-based instruments, making them suitable for a wide range of molecular biology applications, including PCR, RT-PCR, and NGS.

By integrating magnetic bead technology, the InviMag® product line provides a reliable and efficient solution for nucleic acid purification, meeting the stringent requirements of scientific research and diagnostic workflows.

Key Advantages

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Highest Purity and Yield: Optimized for superior nucleic acid isolation.

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Broad Instrument Compatibility: Compatible with various magnetic bead-based instruments.

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Certified Quality Standards: ISO 13485 certified CE-IVD and RUO kits.

Instrument Compatibility

Some of our InviMag® kits, like InviMag® Universal Kit/IG or the InviMag® Blood DNA Mini Kit/IG, were specifically developed for our own magnetic bead handling instrument – the InviGenius® PLUS. This instrument features fully automated and scalable extraction of DNA and RNA from up to 12 samples. A step-by-step wizard of the InviGenius® PLUS software guides through the method setup for loading the instrument with samples and kit components for nucleic acid extraction. Automatic barcode reading enables full tracking of samples and reagents. Defined and optimized protocols provide high yields and pure nucleic acids.

Most InviMag® kits were developed for the well-established KingFisher™ series from Thermo Fisher Scientific. This instrument series covers instruments for processing 8, 12, 24 or up to 96 samples in parallel. Our InviMag® kits were optimized for the KingFisher™ Flex (up to 96 samples) and the KingFisher™ Duo (up to 2×12 samples) instruments. Specific run protocols for these instruments can be directly downloaded from the individual product pages. For further run protocols contact: techsupport@invitek.com.

Meanwhile, instruments of the Auto-Pure® series from Allsheng are available in numerous laboratories across the world. Our InviMag® kits are compatible with all Auto-Pure® instruments. This instrument series comprises various instruments for low to high-throughput laboratories. Our InviMag® kits were optimized for the Auto-Pure® 96 (up to 96 samples) and the Auto-Pure® Mini (up to 16 samples) instruments. Specific run protocols for these instruments can be directly downloaded from the individual product pages. For further run protocols contact: techsupport@invitek.com.

In general, our InviMag® Kits are compatible with most magnetic bead-based instruments. We offer specific kits without plasticware to allow integration in other systems using different consumables. If you need support with a specific instrument, just contact us: techsupport@invitek.com.